Mastromatteo S, et al.. Beyond reference bias: Making pangenomes accessible with PangyPlot. bioRxiv. 2025.
doi: 10.1101/2025.10.31.684064.
Supporting work
Human Pangenome Reference Consortium
Pangenome reference data source.
Human Pangenome Reference Consortium. A draft human pangenome reference. Nature. 2023 May;617(7960):312-324.
doi: 10.1038/s41586-023-05896-x.
BubbleGun
Used for calculating graph hierarchical structure.
Dabbaghie F, Ebler J, Marschall T. BubbleGun: enumerating bubbles and superbubbles in genome graphs. Bioinformatics. 2022 Sep;38(17):4217-4219.
doi: 10.1093/bioinformatics/btac448.
odgi layout
Used for generating the initial 2D layout.
Guarracino A, Heumos S, Nahnsen S, Prins P, Garrison E. ODGI: understanding pangenome graphs. Bioinformatics. 2022 Jul;38(13):3319-3326.
doi: 10.1093/bioinformatics/btac308.
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Welcome to PangyPlot
PangyPlot is a tool designed for visualizing graph-based pangenome data. Currently, PangyPlot is set to
visualize data from the HPRC pangenome graph. [Minigraph-Cactus, GRCh38 Graph, clip] with GENCODE annotations.
To quickly run, select an "Example" button at the top of the page. Or input your region of interest then
click "Go".
As PangyPlot is still undergoing development, please treat this instance as a public beta.
Bug fixes and feature additions are ongoing.
If you have a particular region of interest and need help, with visualization, please reach out to scott.mastromatteo@sickkids.ca.
You can also report issues on our GitHub Issues page.
The tool is provided as-is and may be updated or withdrawn without notice.
This tool is intended for research and educational purposes only. Outputs are not intended for clinical, diagnostic, or therapeutic use.